Article
Quantitative phenotyping of X-disease resistance in chokecherry using real-time PCR.
Journal of microbiological methods - 1 Mar 2014
Huang Danqiong, Walla James A, Dai Wenhao
Abstract excerpt
A quantitative real-time SYBR Green PCR (qPCR) assay has been developed to detect and quantify X-disease phytoplasmas in chokecherry. An X-disease phytoplasma-specific and high sensitivity primer pair was designed based on the 16S rRNA gene sequence of X-disease phytoplasmas. This primer pair was specific to the 16SrIII group (X-disease) phytoplasmas. The qPCR method can quantify phytoplasmas from a DNA mix (a...
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