Article
[Study on identification of Astragali Radix and Hedysari Radix by PCR amplification of specific alleles].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Aug 2013
Long Ping, Cui Zhan-Hu, Li Qian-Quan, Xu Jian-Ping, Zhang Chun-Hong, Zhou Li-She, Li Min-Hui
Abstract excerpt
To explore the new method of discriminating Astragali Radix and Hedysari Radix by using PCR amplification of specific alleles, 30 samples of the different Astragali Radix materials and 28 samples of Hedysari Radix were collected. The total DNA of all samples were extracted, trnL-trnF sequence from Astragali Radix and Hedysari Radix was amplified by PCR and sequenced unidirectionally. These sequences were aligned...
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