Article
[Authenticate of Atractylodes macrocephala seed by amplification refractory mutation system].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Aug 2013
Cao Liang, Jiang Chao, Peng Hua-Sheng, Chen Min, Yuan Yuan
Abstract excerpt
OBJECTIVE: To design specific primers and authenticate Atractylodes macrocephala from Atractylodes lancea and A. chinensis. METHOD: SNPs in the psbA-trnH sequences of Atractylodes were found by ClustulW program and Bioedit software. Primers for authentic A. macrocephala is designed according to the SNP site, and ITS sequence universal primers plus to the authentic primer to construct a multi-PCR reaction system,...
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