Article
[Identification of Bletillae Rhizoma and its adulterants by SNPs in ITS2].
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica - 1 Sept 2015
Zhao Dan, Zhou Tao, Jiang Wei-ke, Xiao Cheng-hong, Kang Chuan-zhi
Abstract excerpt
To establish a molecular identification method for Bletillae Rhizoma, this paper extracted genome DNA from Bletillae Rhizoma and its adulterants. The sequences of rDNA ITS2 were sequenced after amplifying. Then multiple alignments of ITS2 were constructed phylogenetic tree with Neighbor Joining by MEGA 5. 1 and found out SNPs loci. The result showed that rDNA ITS2 region could identify Bletillae Rhizoma and its...
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