Article
Two glycosylated vacuolar GFPs are new markers for ER-to-vacuole sorting.
Plant physiology and biochemistry : PPB - 1 Dec 2013
Stigliano Egidio, Faraco Marianna, Neuhaus Jean-Marc, Montefusco Anna, Dalessandro Giuseppe, Piro Gabriella, Di Sansebastiano Gian-Pietro
Abstract excerpt
Vacuolar Sorting Determinants (VSDs) have been extensively studied in plants but the mechanisms for the accumulation of storage proteins in somatic tissues are not yet fully understood. In this work we used two mutated versions of well-documented vacuolar fluorescent reporters, a GFP fusion in frame with the C-terminal VSD of tobacco chitinase (GFPChi) and an N-terminal fusion in frame with the sequence-specific...
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