Article
In vitro transcription of the plasma protein genes of Bombyx mori.
Biochimica et biophysica acta - 10 Sept 1990
Sakurai H, Izumi S, Tomino S
Abstract excerpt
An efficient cell-free transcription system was developed from the extract of BmN cells established from an ovarian tissue of the silkworm, Bombyx mori. The cloned genes coding for major plasma proteins of B. mori including SP 1, SP 2 and 30K protein, were faithfully and efficiently transcribed in the extract prepared from BmN cells. The S1 nuclease mapping and primer extension analyses demonstrated that the...
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