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IMAC Fractionation in Combination with LC–MS Reveals H2B and NIF-1 Peptides As Potential Bladder Cancer Biomarkers

7 Aug 2013

Abstract excerpt

Improvement in bladder cancer (BC) management requires more effective diagnosis and prognosis of disease recurrence and progression. Urinary biomarkers attract special interest because of the noninvasive means of urine collection. Proteomic analysis of urine entails the adoption of a fractionation methodology to reduce sample complexity. In this study, we applied immobilized metal affinity chromatography in...

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PMID: 23924207
DOI: 10.1021/pr400255h

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