Article
Identification and isolation of small CD44-negative mesenchymal stem/progenitor cells from human bone marrow using elutriation and polychromatic flow cytometry.
Stem cells translational medicine - 1 Aug 2013
Hall Sean R R, Jiang Yajuan, Leary Elizabeth, Yavanian Greg, Eminli Sarah, O'Neill David W, Marasco Wayne A
Abstract excerpt
The method of isolation of bone marrow (BM) mesenchymal stem/stromal cells (MSCs) is a limiting factor in their study and therapeutic use. MSCs are typically expanded from BM cells selected on the basis of their adherence to plastic, which results in a heterogeneous population of cells. Prospective identification of the antigenic profile of the MSC population(s) in BM that gives rise to cells with MSC activity in...
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