Article
Large-scale resource development in Gossypium hirsutum L. by 454 sequencing of genic-enriched libraries from six diverse genotypes.
Plant biotechnology journal - 1 Oct 2013
Rai Krishan Mohan, Singh Sunil Kumar, Bhardwaj Archana, Kumar Verandra, Lakhwani Deepika, Srivastava Apeksha, Jena Satya Narayan, Yadav Hemant Kumar, Bag Sumit K, Sawant Samir V
Abstract excerpt
The sequence information has been proved to be an essential genomic resource in case of crop plants for their genetic improvement and better utilization by humans. To dissect the Gossypium hirsutum genome for large-scale development of genomic resources, we adopted hypomethylated restriction-based genomic enrichment strategy to sequence six diverse genotypes. Approximately 5.2-Gb data (more than 18.36 million...
Topics
- DNA, Plant
- Databases, Genetic
- Gene Library
- Genetic Markers
- Genotype
- Gossypium
- Microsatellite Repeats
- Molecular Sequence Annotation
- Polymorphism, Single Nucleotide
- Sequence Analysis, DNA