Article
Phosphorylated variant of bovine prolactin.
Molecular and cellular endocrinology - 18 Jun 1990
Brooks C L, Kim B G, Aphale P, Kleeman B E, Johnson G C
Abstract excerpt
Bovine pituitary explants and cell cultures were incubated with [32P]orthophosphate. Extracts were prepared from the explants and analyzed by sodium dodecyl sulfate-containing acrylamide gel electrophoresis and autoradiography revealing a phosphoprotein that co-migrated with authentic bovine prolactin. Clonal antibodies to bovine prolactin were produced, purified and used to prepare affinity columns. Extracts of...
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