Article
Development of an optimized medium, strain and high-throughput culturing methods for Methylobacterium extorquens.
PloS one - 1 Jan 2013
Delaney Nigel F, Kaczmarek Maria E, Ward Lewis M, Swanson Paige K, Lee Ming-Chun, Marx Christopher J
Abstract excerpt
Methylobacterium extorquens strains are the best-studied methylotrophic model system, and their metabolism of single carbon compounds has been studied for over 50 years. Here we develop a new system for high-throughput batch culture of M. extorquens in microtiter plates by jointly optimizing the properties of the organism, the growth media and the culturing system. After removing cellulose synthase genes in M....
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