Article
Primary structures of two protamine 2 variants (St2a and St2b) from stallion spermatozoa.
Biochimica et biophysica acta - 19 Jun 1990
Pirhonen A, Valtonen P, Linnala-Kankkunen A, Heiskanen M L, Mäenpää P H
Abstract excerpt
Protamines were extracted from stallion sperm cell nuclei, alkylated with iodoacetamide and separated by reversed-phase high-performance liquid chromatography. Two main components, protamine 1 and protamine 2, were obtained. The latter contains two subspecies, separable by acetic acid-urea-polyacrylamide gel electrophoresis. The primary structure of protamine 2a (St2a) was determined by analysis of fragments...
Topics
- Alkylation
- Amino Acid Sequence
- Animals
- Chromatography, High Pressure Liquid
- Electrophoresis, Polyacrylamide Gel
- Genetic Variation
- Horses
- Iodoacetamide
- Male
- Molecular Sequence Data
- Protamines