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Article

A transgenic medaka line with visible markers for genotypic and phenotypic sex.

Environmental science & technology - 18 Jun 2013

Kanamori Akira, Toyama Keiko

Abstract excerpt

Accurate genotyping of sex is required for correct interpretation in any in vivo assays with endocrine disrupting chemicals (EDCs). Visible markers for genotypic sex, if reliable, simplify assays because time-consuming PCR-based genotyping can be skipped. Here, we describe a line of Japanese medaka with a brain-expressed green fluorescent protein (GFP) transgene inserted near the sex-determining locus. When used...

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PMID: 23638909
DOI: 10.1021/es400264q

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