Article
[The use of filamentous phage M13 in protein engineering].
Molekuliarnaia biologiia - 1 Jan 2000
Il'ichev A A, Minenkova O O, Tat'kov S I, Karpyshev N N, Eroshkin A M, Ofitserov V I, Akimenko Z A, Petrenko V A, Sandakhchiev L S
Abstract excerpt
M13B1 vector based on the filamentous phage M13 has been constructed. M13B1 phage carries the gene of resistance to ampicillin and contains the unique site of recognition for BamHI restriction endonuclease in gene VIII coding for the major coat protein. BamHI restriction site has been inserted in...
Topics
- Amino Acid Sequence
- Base Sequence
- Blotting, Southern
- Capsid
- Capsid Proteins
- Coliphages
- DNA, Viral
- Membrane Proteins
- Molecular Sequence Data
- Mutation
- Protein Engineering
- Recombination, Genetic
