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Development of porcine rotavirus vp6 protein based ELISA for differentiation of this virus and other viruses

22 Mar 2013

Abstract excerpt

BACKGROUND: The context and purpose of the study included 1) bacterial expression of viral protein 6 (VP6) of porcine rotavirus (PRV) and generation of rabbit polyclonal antiserum to the VP6 protein; 3) establishment of a discrimination ELISA to distinguish PRV from a panel of other porcine viruses. RESULTS: The VP6 gene of PRV isolate DN30209 amplified by reverse transcription-PCR was 1356 bp containing a...

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PMID: 23517810
DOI: 10.1186/1743-422x-10-91

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