Article
[Study on ITS sequences of Aconitum vilmorinianum and its medicinal adulterant].
Zhong yao cai = Zhongyaocai = Journal of Chinese medicinal materials - 1 Sept 2012
Zhang Xiao-nan, Du Chun-hua, Fu De-huan, Gao Li, Zhou Pei-jun, Wang Li
Abstract excerpt
OBJECTIVE: To analyze and compare the ITS sequences of Aconitum vilmorinianum and its medicinal adulterant Aconitum austroyunnanense. METHODS: Total genomic DNA were extracted from sample materials by improved CTAB method, ITS sequences of samples were amplified using PCR systems, directly sequenced and analyzed using software DNAStar, ClustalX1.81 and MEGA 4.0. RESULTS: 299 consistent sites, 19 variable sites...
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