Article
Orthology Guided Assembly in highly heterozygous crops: creating a reference transcriptome to uncover genetic diversity in Lolium perenne.
Plant biotechnology journal - 1 Jun 2013
Ruttink Tom, Sterck Lieven, Rohde Antje, Bendixen Christian, Rouzé Pierre, Asp Torben, Van de Peer Yves, Roldan-Ruiz Isabel
Abstract excerpt
Despite current advances in next-generation sequencing data analysis procedures, de novo assembly of a reference sequence required for SNP discovery and expression analysis is still a major challenge in genetically uncharacterized, highly heterozygous species. High levels of polymorphism inherent...
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