Article
Porcine skeletal muscle differentially expressed gene ATP5B: molecular characterization, expression patterns, and association analysis with meat quality traits.
Mammalian genome : official journal of the International Mammalian Genome Society - 1 Apr 2013
Xu Haixia, Xu Yongjie, Liang Xiaojuan, Wang Yanbo, Jin Fangfang, Liu Dengying, Ma Yun, Yuan Hongyu, Song Xinqiang, Zeng Wenxian
Abstract excerpt
The 2-DE/MS-based proteomics approach was used to investigate the differences of porcine skeletal muscle, and ATP5B was identified as one differential expression protein. In the present study, ATP5B gene was further cloned by RT-PCR, the sequence was analyzed using the bioinformatics method, and the mRNA expression was detected by qRT-PCR. Sequence analysis showed that the porcine ATP5B gene contains an ORF...
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