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Article

Optimization of in vitro HBV replication and HBsAg production in HuH7 cell line.

Journal of virological methods - 1 Apr 2013

Cavallone Daniela, Moriconi Francesco, Colombatto Piero, Oliveri Filippo, Bonino Ferruccio, Brunetto Maurizia Rossana

Abstract excerpt

The Gunther's vector-free method (GM), using PCR-amplified full length HBV-DNA (fl-HBV-DNA), is currently the best in vitro HBV replication system despite the low intracellular HBV-DNA production. The replication efficiency and HBsAg secretion of 12 isolates from HBsAg/HBeAg positive sera by GM, Monomer-Linear-Sticky-Ends-DNA (MLSE) and Monomer-Circular-Closed (MCC) were compared in HuH7 cells. Eight of twelve...

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Share to DNA, Circular
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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Share to Genome, Viral

Share to Hepatitis B e Antigens

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Source

Identifiers

PMID: 23391821
DOI: 10.1016/j.jviromet.2013.01.012

Genes

MCC

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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