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Article

Improved allele-specific PCR technique for Kidd blood group genotyping.

Journal of clinical laboratory analysis - 1 Jan 2013

Intharanut Kamphon, Grams Rudi, Bejrachandra Sasitorn, Sriwanitchrak Pramote, Nathalang Oytip

Abstract excerpt

BACKGROUND: We developed an allele-specific polymerase chain reaction (AS-PCR) technique for Kidd blood group genotyping. METHODS: Altogether, 340 blood samples from Thai blood donors at the National Blood Centre, Thai Red Cross Society, were tested with anti-Jk(a) and anti-Jk(b) using the gel technique and the direct urea lysis test was used for screening Jk(a-b-) phenotype. For AS-PCR technique, different types...

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PMID: 23325744
DOI: 10.1002/jcla.21561
PMCID: PMC6807600

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