Article
QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L.
PloS one - 1 Jan 2013
Zou Zhongwei, Ishida Masahiko, Li Feng, Kakizaki Tomohiro, Suzuki Sho, Kitashiba Hiroyasu, Nishio Takeshi
Abstract excerpt
SNP markers for QTL analysis of 4-MTB-GSL contents in radish roots were developed by determining nucleotide sequences of bulked PCR products using a next-generation sequencer. DNA fragments were amplified from two radish lines by multiplex PCR with six primer pairs, and those amplified by 2,880 primer pairs were mixed and sequenced. By assembling sequence data, 1,953 SNPs in 750 DNA fragments, 437 of which have...
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