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Article

Studying the replication history of human B lymphocytes by real-time quantitative (RQ)-PCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2013

van Zelm Menno C, Berkowska Magdalena A, van Dongen Jacques J M

Abstract excerpt

The cells of the adaptive immune system, B and T lymphocytes, each generate a unique antigen receptor through V(D)J recombination of their immunoglobulin (Ig) and T cell receptor (TCR) loci, respectively. Such rearrangements join coding elements to form a coding joint and delete the intervening DNA as circular excision products containing the signal joint. These excision circles are stable structures that cannot...

Topics

Alleles
B-Lymphocytes
Cell Proliferation
Cell Separation
DNA
Humans
Real-Time Polymerase Chain Reaction
Taq Polymerase

Source

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Identifiers

PMID: 23296960
DOI: 10.1007/978-1-62703-269-8_6

Genes

IGK

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 30 Aug 2026.

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