Article
Application of DNA sequence analysis based on five different conserved genes (16S rDNA, rpoB, gdh, est and pgm) for intra-species discrimination of Bacteroides fragilis.
Anaerobe - 1 Feb 2013
Miranda Karla Rodrigues, Neves Felipe Piedade Gonçalves, Santos-Filho Joaquim dos, de Paula Geraldo Renato, Lobo Leandro Araújo, Oelemann Walter Martin Roland, Domingues Regina Maria Cavalcanti Pilotto
Abstract excerpt
In the past few years, many studies revealed a remarkable genetic variability in Bacteroides fragilis species, and the existence of two divisions was proposed according to presence or absence of the cfiA (metallo-β-lactamase/carbapenemase) gene. The aim of this study was to evaluate the use of DNA sequence analysis for glutamate dehydrogenase (gdh), phosphoglucomutase (pgm) and esterase (est) metabolic genes, in...
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