Article
Species-specific real-time PCR detection of Colletotrichum kahawae.
Journal of applied microbiology - 1 Mar 2013
Tao G, Hyde K D, Cai L
Abstract excerpt
AIMS: Colletotrichum kahawae is a strongly aggressive pathogen causing coffee berry disease and is specific to Arabica coffee (Coffea arabica) in Africa. In this article, we developed a real-time PCR assay for the species-specific diagnosis of C. kahawae by designing the primers and a TaqMan probe derived from the single nucleotide polymorphism-rich region of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) gene....
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