Article
Diversity patterns of uncultured Haptophytes unravelled by pyrosequencing in Naples Bay.
Molecular ecology - 1 Jan 2013
Bittner Lucie, Gobet Angélique, Audic Stéphane, Romac Sarah, Egge Elianne S, Santini Sébastien, Ogata Hiroyuki, Probert Ian, Edvardsen Bente, de Vargas Colomban
Abstract excerpt
Haptophytes are a key phylum of marine protists, including ~300 described morphospecies and 80 morphogenera. We used 454 pyrosequencing on large subunit ribosomal DNA (LSU rDNA) fragments to assess the diversity from size-fractioned plankton samples collected in the Bay of Naples. One group-specific primer set targeting the LSU rDNA D1/D2 region was designed to amplify Haptophyte sequences from nucleic acid...
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