Article
Sexing frogs by real-time PCR: using aromatase (cyp19) as an early ovarian differentiation marker.
Sexual development : genetics, molecular biology, evolution, endocrinology, embryology, and pathology of sex determination and differentiation - 1 Jan 2012
Navarro-Martín L, Velasco-Santamaría Y M, Duarte-Guterman P, Robertson C, Lanctôt C, Pauli B, Trudeau V L
Abstract excerpt
Most anurans have no identified sex-markers; therefore, alternative methods for identification of early changes in sex ratios are required. In this study, Lithobates sylvaticus and Silurana tropicalis tadpoles were sampled at different developmental stages covering the entire process of sex differentiation. Three candidate genes known to be involved in sex differentiation in other vertebrate species were selected...
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