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Biochemical and functional characterization of human phospholipid scramblase 4 (hPLSCR4).

Biological chemistry - 1 Oct 2012

Francis Vincent Gerard, Gummadi Sathyanarayana N

Abstract excerpt

Human phospholipid scramblase 4 (hPLSCR4), an isoform of the scramblase family, is a type II single-pass transmembrane protein whose function remains unknown. To understand its role, recombinant hPLSCR4 was obtained by cloning the ORF into a pET28 a(+) vector and overexpressed in Escherichia coli. Functional assay showed that Ca2+, Mg2+, and Zn2+ activate hPLSCR4 and mediate scrambling activity independent of the...

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Calcium
Hydrophobic and Hydrophilic Interactions
Mutagenesis, Site-Directed
Mutation
Phospholipid Transfer Proteins

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Spectrometry, Fluorescence

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PMID: 23089641
DOI: 10.1515/hsz-2012-0129

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