Article
Analysis of site-directed mutations in the alpha- and beta-subunits of Klebsiella pneumoniae nitrogenase.
Molecular microbiology - 1 Sept 1990
Kent H M, Baines M, Gormal C, Smith B E, Buck M
Abstract excerpt
Using directed mutagenesis, amino acid substitutions have been made in the alpha- and beta-subunits of the klebsiella pneumoniae nitrogenase component 1 at positions normally occupied by conserved cysteine or tyrosine residues. Nif+, Nif- and intermediate phenotypes have been obtained. To extend...
Topics
- Electrophoresis, Polyacrylamide Gel
- Immunoblotting
- Klebsiella pneumoniae
- Molybdoferredoxin
- Mutagenesis, Site-Directed
- Nitrogenase
- Phenotype