Article
Detection and serotyping of lyophilized nonculturable pneumococcal isolates.
Journal of clinical microbiology - 1 Oct 2012
Modak Joyanta K, Steinhoff Mark C, Zaman K, Islam Maksuda, El Arifeen Shams, Saha Samir K
Abstract excerpt
One hundred fifty-two nonculturable lyophilized carriage pneumococcal isolates from a vaccine trial were subjected to PCR for serotyping, and 149 (98%) were successfully classified as vaccine or nonvaccine types, which were similar to viable isolates. The methodology will be useful for analysis of this and other studies where stored pneumococcal isolates fail to grow.
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