Article
Rapid genotyping of Pseudomonas aeruginosa isolates harboured by adult and paediatric patients with cystic fibrosis using repetitive-element-based PCR assays.
Journal of medical microbiology - 1 Nov 2004
Syrmis Melanie W, O'Carroll Mark R, Sloots Theo P, Coulter Chris, Wainwright Claire E, Bell Scott C, Nissen Michael D
Abstract excerpt
In this study, the suitability of two repetitive-element-based PCR (rep-PCR) assays, enterobacterial repetitive intergenic consensus (ERIC)-PCR and BOX-PCR, to rapidly characterize Pseudomonas aeruginosa strains isolated from patients with cystic fibrosis (CF) was examined. ERIC-PCR utilizes paired sequence-specific primers and BOX-PCR a single primer that target highly conserved repetitive elements in the P....
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