Article
Accurate and rapid species typing from cutaneous and mucocutaneous leishmaniasis lesions of the New World.
Diagnostic microbiology and infectious disease - 1 Oct 2012
Fraga Jorge, Veland Nicolas, Montalvo Ana M, Praet Nicolas, Boggild Andrea K, Valencia Braulio M, Arévalo Jorge, Llanos-Cuentas Alejandro, Dujardin Jean-Claude, Van der Auwera Gert
Abstract excerpt
The heat-shock protein 70 gene (hsp70) has been exploited for Leishmania species identification in the Old and New World, using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis. Three new Leishmania-specific hsp70 PCRs were recently described, and we applied 2 of these on 89 clinical samples from a total of 73 Peruvian patients with either cutaneous or mucocutaneous...
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