Article
Novel FixL homologues in Chlamydomonas reinhardtii bind heme and O(2).
FEBS letters - 14 Dec 2012
Murthy U M Narayana, Wecker Matt S A, Posewitz Matthew C, Gilles-Gonzalez Marie-Alda, Ghirardi Maria L
Abstract excerpt
Genome inspection revealed nine putative heme-binding, FixL-homologous proteins in Chlamydomonas reinhardtii. The heme-binding domains from two of these proteins, FXL1 and FXL5 were cloned, expressed in Escherichia coli, purified and characterized. The recombinant FXL1 and FXL5 domains stained positively for heme, while mutations in the putative ligand-binding histidine FXL1-H200S and FXL5-H200S resulted in loss...
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