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Development and evaluation of one step single tube multiplex RT-PCR for rapid detection and typing of dengue viruses

30 Jan 2008

Abstract excerpt

BACKGROUND: Dengue is emerging as a major public health concern in many parts of the world. The development of a one-step, single tube, rapid, and multiplex reverse transcription polymerase chain reaction (M-RT-PCR) for simultaneous detection and typing of dengue virus using serotype specific primers during acute phase of illness is reported. RESULTS: An optimal assay condition with zero background was...

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PMID: 18234069
DOI: 10.1186/1743-422x-5-20

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