Article
A computer program for selection of oligonucleotide primers for polymerase chain reactions.
Nucleic acids research - 11 Apr 1990
Lowe T, Sharefkin J, Yang S Q, Dieffenbach C W
Abstract excerpt
We have designed a computer program which rapidly scans nucleic acid sequences to select all possible pairs of oligonucleotides suitable for use as primers to direct efficient DNA amplification by the polymerase chain reaction. This program is based on a set of rules which define in generic terms both the sequence composition of the primers and the amplified region of DNA. These rules (1) enhance primer-to-target...
Topics
- Algorithms
- Base Sequence
- Endothelin-1
- Eye Neoplasms
- Gene Amplification
- Genetic Predisposition to Disease
- Humans
- Molecular Sequence Data
- Oligonucleotide Probes
- Peptides
- Polymerase Chain Reaction