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Handmade Cloned Transgenic Piglets Expressing the Nematode Fat-1 Gene

1 Jun 2012

Abstract excerpt

Production of transgenic animals via somatic cell nuclear transfer (SCNT) has been adapted worldwide, but this application is somewhat limited by its relatively low efficiency. In this study, we used handmade cloning (HMC) established previously to produce transgenic pigs that express the functional nematode fat-1 gene. Codon-optimized mfat-1 was inserted into eukaryotic expression vectors, which were transferred...

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PMID: 22686479
DOI: 10.1089/cell.2011.0073

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