Article
[Construction and sequence analysis of a drought-induced full-length cDNA library from Ammopiptanthus mongolicus].
Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Jan 2012
Lin Qingfang, Wang Xuefeng, Li Jiyuan, Zhao Hongbin, Wang Maoyan
Abstract excerpt
Ammopiptanthus mongolicus shows very strong resistance to severe environments. To isolate drought-resistant genes and elucidate drought-resistant molecular mechanisms of the plant, we constructed a drought-induced full-length cDNA library using SMART (Switching mechanism at 5'-end of RNA transcript) technique. The phage titer of the unamplified library was 1.6 x 10(7) PFU/mL; the recombination percentage was...
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