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Article

Comparative analysis of gene expression level by quantitative real-time PCR has limited application in objects with different morphology.

PloS one - 1 Jan 2012

Demidenko Natalia V, Penin Aleksey A

Abstract excerpt

qRT-PCR is a generally acknowledged method for gene expression analysis due to its precision and reproducibility. However, it is well known that the accuracy of qRT-PCR data varies greatly depending on the experimental design and data analysis. Recently, a set of guidelines has been proposed that aims to improve the reliability of qRT-PCR. However, there are additional factors that have not been taken into...

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PMID: 22666472
DOI: 10.1371/journal.pone.0038161
PMCID: PMC3364230

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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