Article
Chloroplast subspecies-specific SNP detection and its maternal inheritance in Brassica oleracea L. by using a dCAPS marker.
The Journal of heredity - 1 Jul 2012
Zhang Yangyong, Fang Zhiyuan, Wang Qingbiao, Liu Yumei, Yang Limei, Zhuang Mu, Sun Peitian
Abstract excerpt
Chloroplast simple sequence repeats amplicons in 5 subspecies of Brassica oleracea were sequenced, and one chloroplast SNP was detected in amplicon ACP43. Through the introduction of an RsaI recognition site by adding one mismatch in the forward primer, combined with the increased primer length and raised annealing temperature, the dCAPS (derived cleaved amplified polymorphic sequences) marker ACP43-93 RsaI was...
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