Article
Mutagenic effect of freezing on nuclear DNA of Saccharomyces cerevisiae.
Yeast (Chichester, England) - 1 May 2012
Todorova T, Pesheva M, Stamenova R, Dimitrov M, Venkov P
Abstract excerpt
Although fragmentation of DNA has been observed in cells undergoing freezing procedures, a mutagenic effect of sub-zero temperature treatment has not been proved by induction and isolation of mutants in nuclear DNA (nDNA). In this communication we supply evidence for mutagenicity of freezing on nDNA of Saccharomyces cerevisiae cells. In the absence of cryoprotectors, cooling for 2 h at +4°C and freezing for 1 h...
Topics
- Cell Nucleus
- DNA, Fungal
- Freezing
- Mutagens
- Mutation
- Reactive Oxygen Species
- Saccharomyces cerevisiae