Article
RFLP analysis of cry1 and cry2 genes of Bacillus thuringiensis isolates from India.
Journal of microbiology and biotechnology - 1 Jun 2012
Patel Ketan D, Ingle Sanjay S
Abstract excerpt
The PCR-RFLP method has been useful for detection of known genes and identification of novel genes. In the present study, degenerate primers were designed from five groups of cry1 genes for PCR-RFLP analysis. Bacillus thuringiensis (Bt) isolates from different regions were evaluated for PCR amplification of various cry1 genes using newly designed primers and cry2 genes using reported primers. PCR analysis showed...
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