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Article

Wnt5a participates in hepatic stellate cell activation observed by gene expression profile and functional assays

1 Jan 2012

Abstract excerpt

AIM: To identify differentially expressed genes in quiescent and activated hepatic stellate cells (HSCs) and explore their functions. METHODS: HSCs were isolated from the normal Sprague Dawley rats by in suit perfusion of collagenase and pronase and density Nycodenz gradient centrifugation. Total RNA and mRNA of quiescent HSCs, and culture-activated HSCs were extracted, quantified and reversely transcripted into...

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PMID: 22553398
DOI: 10.3748/wjg.v18.i15.1745

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