Article
Development of a species-specific sequence-characterized amplified region marker for roses.
Genetics and molecular research : GMR - 24 Feb 2012
Riaz S, Sadia B, Awan F S, Khan I A, Sadaqat H A, Khan I A
Abstract excerpt
DNA fingerprints of four rose species, Rosa centifolia, R. Gruss-an-Teplitz, R. bourboniana, and R. damascena, were developed using RAPD-PCR. We identified a unique polymorphic band in R. centifolia. This 762-bp fragment was produced by the random primer GLI-2. The fragment was eluted and directly cloned in a TA cloning vector, pTZ57R/T. Digestion of the plasmid with EcoRI confirmed the cloning of...
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