Article
Human smooth muscle VLA-1 integrin: purification, substrate specificity, localization in aorta, and expression during development.
The Journal of cell biology - 1 Nov 1990
Belkin V M, Belkin A M, Koteliansky V E
Abstract excerpt
A membrane glycoprotein complex was isolated and purified from human smooth muscle by detergent solubilization and affinity chromatography on collagen-Sepharose. The complex was identified as VLA-1 integrin and consisted of two subunits of 195 and 130 kD in SDS-PAGE. Liposomes containing the VLA-1 integrin adhered to surfaces coated with type I, II, III, and IV collagens, Clq subcomponent of the first component...
Topics
- Aorta
- Cell Compartmentation
- Cells, Cultured
- Fluorescent Antibody Technique
- Gene Expression Regulation
- Humans
- Ligands
- Microscopy, Fluorescence
- Muscle, Smooth, Vascular
- Organ Specificity
- Phenotype