Article
Locus assignment of human alpha-globin structural mutants by selective enzymatic amplification of alpha 1 and alpha 2-globin cDNAs.
Human genetics - 1 Oct 1990
Mamalaki A, Horanyi M, Szelenyi J, Moschonas N K
Abstract excerpt
We have used the powerful methodology of DNA enzymatic amplification in order to assign human alpha-globin structural mutants to one of the two highly homologous alpha-globin genes. Selectively amplified alpha 1 and alpha 2-globin cDNAs were dot-blotted and further hybridized to synthetic oligonucleotides encompassing either the normal or the mutated sequences. The generated signals corresponded specifically to...
Topics
- Base Sequence
- Chromosome Mapping
- DNA
- DNA Mutational Analysis
- Gene Amplification
- Genes
- Globins
- Humans
- Immunoblotting
- Molecular Sequence Data
- Mutation