Article
Simple Nested Allele-Specific approach with penultimate mismatch for precise species and sex identification of tiger and leopard.
Molecular biology reports - 1 Feb 2021
Nittu George, Bhavana Pudupet Madhavan, Shameer Thekke Thumbath, Ramakrishnan Balasundaram, Archana Rajan, Kaushal Krishan Kumar, Khedkar Gulab Dattarao, Mohan Govindarajan, Jyothi Manikkiri, Sanil Raveendranathanpillai
Abstract excerpt
Accurate species and sex identification of non-invasive and forensic samples of the tiger and leopard is still confusing when using the allele-specific methods. We designed allele-specific methods with penultimate nucleotide mismatch in a nested manner for the exact identification and double-checking of forensic samples. The mismatch design is a novel concept in species and sex identification, making the...
Topics
- Alleles
- Animals
- Biomarkers
- DNA Primers
- Endangered Species
- Female
- Forensic Genetics
- Gender Identity
- India
- Male
- Panthera
- Polymerase Chain Reaction
- Sensitivity and Specificity