Article
[Identification of Daturae flos and its adulterants based on DNA barcoding technique].
Yao xue xue bao = Acta pharmaceutica Sinica - 1 Nov 2011
Han Jian-ping, Li Mei-ni, Luo Kun, Liu Mei-zi, Chen Xiao-chen, Chen Shi-lin
Abstract excerpt
To identify the original plant of Daturae Flos from its adulterants by DNA barcoding, the sequences of ITS2, psbA-trnH, matK, rbcL of four species including Datura metel, Darura innoxia, Darura stramonium and Brugmansia arborea were compared and analyzed. The PCR and sequencing success rate of the four regions (ITS2, psbA-trnH, matK, rbcL) was 100%, 90%, 100% and 85%, respectively. Sequences were assembled with...
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