Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae).

doi:10.1007/s10529-012-0852-4

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Article

Identification and validation of sex-linked SCAR markers in dioecious Hippophae rhamnoides L. (Elaeagnaceae).

Biotechnology letters - 1 May 2012

Korekar Girish, Sharma Ram Kumar, Kumar Rahul, Meenu, Bisht Naveen C, Srivastava Ravi B, Ahuja Paramvir Singh, Stobdan Tsering

Abstract excerpt

The actinorhizal plant seabuckthorn (Hippophae rhamnoides L., Elaeagnaceae) is a wind pollinated dioecious crop. To distinguish male genotypes from female genotypes early in the vegetative growth phase, we have developed robust PCR-based marker(s). DNA bulk samples from 20 male and 20 female plants each were screened with 60 RAPD primers. Two primers, OPA-04 and OPT-06 consistently amplified female-specific (FS)...

Topics

DNA Primers
Genotype
Hippophae
Molecular Sequence Data
Polymorphism, Genetic
Random Amplified Polymorphic DNA Technique
Sequence Analysis, DNA
Sex

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PMID: 22245920
DOI: 10.1007/s10529-012-0852-4

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