Article
Isolation and characterization of a soluble and thermostable phosphite dehydrogenase from Ralstonia sp. strain 4506.
Journal of bioscience and bioengineering - 1 Apr 2012
Hirota Ryuichi, Yamane Sho-taro, Fujibuchi Tatsuya, Motomura Kei, Ishida Takenori, Ikeda Takeshi, Kuroda Akio
Abstract excerpt
Phosphite dehydrogenase (PtxD), which catalyzes the nearly irreversible oxidation of phosphite to phosphate with the concomitant reduction of NAD(+) to NADH, has great potential for NADH regeneration in industrial biocatalysts. Here, we isolated a soil bacterium, Ralstonia sp. strain 4506, that grew at 45°C on a minimal medium containing phosphite as the sole source of phosphorus. A recombinant PtxD of Ralstonia...
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