Article
[Function of rmlB in the pathogenic Escherichia coli 44277 (O2: k1)].
Wei sheng wu xue bao = Acta microbiologica Sinica - 1 Sept 2011
Ding Weiping, Luo Yuanming, Ai Guomin, Jin Cheng
Abstract excerpt
OBJECTIVE: To identify the role of rmlB in synthesizing L-rhamnose in the pathogenic Escherichia coli 44277 (O2:K1:H4). METHODS: The rmlB gene was expressed and the activity of the recombinant protein was assayed by measuring the quantity of reaction product. The rmlB gene was deleted by homologous recombination, then phenotypic changes of the delta rmlB mutant was analyzed by Electron Microscope, Tricine...
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