Article
Rapid isolation and cross-amplification of microsatellite markers in Plectritis congesta (Valerianaceae) with 454 sequencing.
American journal of botany - 1 Dec 2011
McEwen Jamie R, Vamosi Jana C, Rogers Sean M
Abstract excerpt
PREMISE OF THE STUDY: Microsatellite markers were isolated and characterized in Plectritis congesta for studying the evolution of this highly variable species. METHODS AND RESULTS: We used 454 sequencing of DNA enriched for microsatellite repeats to develop microsatellite markers. This produced 262079 reads with an average length of 324 bp, representing approximately 800 microsatellite regions from which 48...
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